پلی مورفیسم ژن‌های اینترفرون گاما 874+ و اینترلوکین ده 1082- در افراد سالم

  I Abdi Rad, [1] PhD M Bagheri [2] , MSc MD Omrani [3] , PhD H Norouzi Pakzad [4] , MD     Received: 18 Nov, 2008 Accepted: 21 Oct, 2009  Abstract Backgrounds & Aims: Some of polymorphisms are located in the promoter regions or other regulatory sequences of cytokine genes and are associated with increased or decreased amount of cytokines production. The aim of this study was to determine the rate of polymorphisms of IFN-γ +874 and IL-10 -1082 in the normal population.  Materials & Methods: 109 normal individuals were included in the study and genomic description was carried out using ASO-PCR method. Results: Allelic frequency of alleles A and T for IFN-γ +874 were 50.92% and 49.08% respectively, and the highest genotype frequency was for the genotype A/T (54.13%). Allelic frequency of A and G for IL-10 -1082 also were 50.92% and 49.08% respectively, and genotype A/G had the highest rate among different genotypes of IL-10 -1082 (96.33%). Conclusion: The alleles A and T of IFN-γ gene (+874) were in Hardy-Weinberg equilibrium, while it was not the case for A and G alleles of IL-10 gene (-1082). Keywords: Polymorphism, Interferon-γ +874, Interleukin-10 -1082   Address: Department of Genetics, Motahhari Hospital, Urmia University of Medical Sciences, Urmia, Iran Tel: (+98 441) 2240166    Email: [email protected]  Source: UMJ 2010: 20(4): 338 ISSN: 1027-3727   [1] Associate Professor of Medical Genetics, Urmia University of Medical Sciences, Urmia, Iran (Corresponding Author)  [2] Instructor of Genetics, Urmia University of Medical Sciences, Urmia, Iran  [3] Associate Professor of Genetics, Urmia University of Medical Sciences, Urmia, Iran  [4] General Practitioner, Urmia University of Medical Sciences, Urmia, Iran